Accession Number:

AD1076497

Title:

Validation of Biotechnology for Quantifying the Abundance and Activity of Vinyl-chloride Oxidizers in Contaminated Groundwater

Descriptive Note:

Technical Report,01 Jul 2014,01 Jul 2019

Corporate Author:

The University of Iowa Iowa City United States

Personal Author(s):

• Mattes,Timothy

Report Date:

2018-03-01

Pagination or Media Count:

40.0

Abstract:

The purpose of this guidance document is to provide a detailed technical protocol for conducting Quantitative PCR qPCR and Reverse transcription quantitative PCR RT-qPCR assays for Vinyl chloride VC-oxidizing bacteria in groundwater and sediment samples. Additional guidance on interpreting molecular data in the context of MNA processes and associated statistical analyses will be provided. This guidance document could be useful for site managers interested in utilizing qPCR RT-qPCR technology at their site or by a commercial laboratories interesting in transferring the technology for commercial applications.

Descriptors:

• Biotechnology
• Groundwater
• contamination
• bacteria
• sampling
• sediments
• biodegradation
• assays
• genes
• lessons learned
• statistical analysis
• geochemistry

Subject Categories:

• Water Pollution and Control

Distribution Statement:

APPROVED FOR PUBLIC RELEASE