Saccharomyces cerevisiae (Budding Yeast); Standard Operating Procedure Series: Toxicology (T)
Technical Report,01 Oct 2017,30 Sep 2018
Engineer Research and Development Center Vicksburg
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Engineered Nano Materials ENMs are commercially used in everyday products, including zinc sunscreens and water resistant fabrics and surfaces, but in the future, they may be used in targeted treatment of cancer, printable monitoring systems, and foldable phones. Understanding the effects of ENMs on the environment is crucial for the responsible use of these technologies. The aim of this project is to develop a standard operating procedure SOP for investigating the effects of ENMs on budding yeast Saccharomyces cerevisiae. The ENMs used to develop this protocol were Ag and CdSeZnS. Toxicity was determined using plate assays to analyze the effect of ENMs on the growth cycle, Fun-1 staining assays to understand the effects on cell metabolism, and quantitative reverse transcriptase-based polymerase chain reaction PCR and RNAseq to understand the effects on genetic expression. From plate assays, doubling times, average time spent in lag phase, maximum concentrations were determined and compared between yeast grown in varying concentrations of ENMs, and a yeast grown in a control environment. Fun-1 staining determines the amount of metabolically active cells present in a treated cell culture. RNAseq and Quantitative PCR results, in expression levels of genes, are used to determine potential toxic effects of ENMs.
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