Accession Number:

AD1071869

Title:

Optimization of RNA Body FluID Assays: Differential Co-Extraction for Sperm Analysis and Rapid Direct Lysis for High-Throughput Evidence Screening

Descriptive Note:

Technical Report,13 Jan 2016,13 Sep 2017

Corporate Author:

University of Central Florida Orlando United States

Personal Author(s):

Report Date:

2018-08-09

Pagination or Media Count:

56.0

Abstract:

The aims of the current work were to custom-develop for DFSC casework use i a differential DNARNA co-extraction isolation protocol for the separation and analysis of non-sperm and sperm fractions in sexual assault evidence i.e. prior to DNA profiling and identification of body fluid of origin and ii a rapid RNA direct lysis method for higher throughput screening of forensic biological evidence blood, semen, saliva, vaginal secretions and menstrual blood.

Subject Categories:

  • Medicine and Medical Research
  • Sociology and Law

Distribution Statement:

APPROVED FOR PUBLIC RELEASE