Optimization of RNA Body FluID Assays: Differential Co-Extraction for Sperm Analysis and Rapid Direct Lysis for High-Throughput Evidence Screening
Technical Report,13 Jan 2016,13 Sep 2017
University of Central Florida Orlando United States
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The aims of the current work were to custom-develop for DFSC casework use i a differential DNARNA co-extraction isolation protocol for the separation and analysis of non-sperm and sperm fractions in sexual assault evidence i.e. prior to DNA profiling and identification of body fluid of origin and ii a rapid RNA direct lysis method for higher throughput screening of forensic biological evidence blood, semen, saliva, vaginal secretions and menstrual blood.
- Medicine and Medical Research
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