Matrix-Free Assisted Laser Desorption Ionization Using Metal-Organic Frameworks
Technical Report,01 Oct 2016,30 Sep 2018
ECBC Aberdeen Proving Ground United States
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We studied the ionization mechanism and determined the factors that affect the charge-transfer process during matrix-assisted laser desorptionionization mass spectrometry MALDI-MS. We addressed the binding affinity issue between metal-organic frameworks MOFs and analytes using low-frequency Raman spectroscopy LFRS to determine inter- and intramolecular changes between crystalline and amorphous states of MOFs with different analytes. These analytes were classified as acidic or basic compounds. As soon as we understand the basic properties of MOFanalyte mixtures, we will investigate how to design MOFs that can enhance ionization efficiency for a wide range of compounds during MALDI-MS analysis. This knowledge will be used to design and develop a reliable, functionalized MOF substrate that can desorb biomolecules and selectively capture analytes of interest. We addressed the potential inter- and intramolecular changes for MOFanalyte mixtures using LFRS and MALDI-MS techniques. The MOFs used in the LFRS were UiO-66-COOH and UiO-66-NH2, and the analytes were pyridine, benzoic acid, cytidine, lauric acid, and guanine. More MOFs than those tested by the LFRS technique were used for the MALDI-MS analyses. Most of the MOFs were obtained from internal sources, either through synthesis or by leveraging from already-funded projects that use MOFs.
- Inorganic Chemistry