Contrast Enhancement Of Biological Nanoporous Materials With Zinc Oxide Infiltration For Electron And X-Ray Nanoscale Microscopy
Journal Article - Open Access
Argonne National Laboratory Argonne United States
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We show that using infiltration of ZnO metal oxide can be useful for high resolution imaging of biological samples in electron and X-ray microscopy. The method is compatible with standard fixation techniques that leave the sample dry, such as finishing with super critical CO2 drying, or simple vacuum drying up to 95 deg C. We demonstrate this technique can be applied on tooth and brain tissue samples. We also show that high resolution X-ray tomography can be performed on biological systems using Zn K edge 1s absorption to enhance internal structures, and obtained the first nanoscale 10 KeV X-ray absorption images of the interior regions of a tooth.
- Nuclear Physics and Elementary Particle Physics