Clinical Significance and Mechanistic Insights into Ovarian Cancer Mitochondrial Dysfunction
Technical Report,01 May 2016,30 Apr 2018
The Pennsylvania State University Hershey United States
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Our work addresses the hypothesis that mitochondrial dysfunction plays a role in the etiology and chemoresistance of epithelial ovarian cancers. We are focusing on the role of the fission protein Drp1 in this context. Specifically we discovered that expression of a low molecular weight Drp1 variant is associated with mitochondrial fissionfusion defects. Mass spec and RNA sequencing analysis has revealed that the low molecular weight LMW isoform of Drp1 does not arise as a consequence of alternate transcriptional promoter use, but may be dependent on an alternate variable domain and C-terminal truncation. We are interrogating the role of short Drp1 as a dominant negative fission protein and are investigating its binding affinity to mitochondria and interaction with fission accessory proteins.
- Medicine and Medical Research