Assessment of Chemical Exposure in Multi-animal and Cell Models Using Multiple Omics Platform
Technical Report,30 Sep 2017,29 Sep 2018
VANDERBILT UNIV NASHVILLE TN NASHVILLE United States
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This work supports the U.S. Army Medical Research and Materiel Commands USAMRMC Biotechnology High Performance Computing Software Applications Institute BHSAI in its efforts to construct predictive models to assess liver, kidney and heart damages of chemical toxicants and drugs. To validate the platform, metabolic changes induced by toxicant exposure is supposed to be experimentally identified from the comprehensive analysis of in vivo transcriptomic data collected from the liver, kidney, heart, plasma, and urine of animals. To translate the results obtained in animal models to human, multiple species of animals, rats and guinea pigs, were used. To dissect the primary from the secondary transcriptomic response of liver, kidney and heart cells, resulted from altered metabolism of other organstissues, to toxicant exposure, transcriptomic analysis was performed on primary hepatocytes, renal tubular epithelial cells, cardiacmyocytes originated from rats. We examined toxic effect of mercury chloride and thioacetamide.
- Anatomy and Physiology