Accession Number:

AD1064692

Title:

Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines

Descriptive Note:

Technical Report,01 Sep 2017,31 Aug 2018

Corporate Author:

University of Rhode Island Kingston United States

Personal Author(s):

Report Date:

2018-09-01

Pagination or Media Count:

25.0

Abstract:

The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive T and B cell responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the second project year, subjects were re-consented for genetic analyses. RNA isolation was completed and library preparation for RNA-seq was performed. Pipelines for generation and analysis of single-cell gene expression and T cell receptor data were established. HLA typing was initiated. Project databases were configured and additional data tables were uploaded.

Subject Categories:

  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE