Accession Number:

AD1064692

Title:

Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines

Descriptive Note:

[Technical Report, Annual Report]

Corporate Author:

University of Rhode Island

Personal Author(s):

• Currier, Jeffrey
• Rothman, Alan L.

Report Date:

2018-09-01

Pagination or Media Count:

25

Abstract:

The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive T and B cell responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the second project year, subjects were re-consented for genetic analyses. RNA isolation was completed and library preparation for RNA-seq was performed. Pipelines for generation and analysis of single-cell gene expression and T cell receptor data were established. HLA typing was initiated. Project databases were configured and additional data tables were uploaded.

Descriptors:

• data analysis
• computational biology
• systems biology
• medical personnel
• gene expression
• institutional review board
• b lymphocytes
• databases
• vaccines
• virus diseases
• immunity
• pipelines
• rna sequence analysis
• biology
• department of defense
• engineering
• immunochemistry
• vaccination
• biomedical research
• blood

Subject Categories:

• Medicine and Medical Research
• Pharmacology

Distribution Statement:

[A, Approved For Public Release]