Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines
Technical Report,01 Sep 2017,31 Aug 2018
University of Rhode Island Kingston United States
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The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive T and B cell responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the second project year, subjects were re-consented for genetic analyses. RNA isolation was completed and library preparation for RNA-seq was performed. Pipelines for generation and analysis of single-cell gene expression and T cell receptor data were established. HLA typing was initiated. Project databases were configured and additional data tables were uploaded.
- Medicine and Medical Research