Accession Number:

AD1064691

Title:

Systems Biology of Immune Response to Live and Inactivated Dengue Virus Vaccines

Descriptive Note:

Technical Report,01 Sep 2017,31 Aug 2018

Corporate Author:

The Geneva Foundation Tacoma United States

Personal Author(s):

Report Date:

2018-09-01

Pagination or Media Count:

25.0

Abstract:

The objective of this project is to elucidate the immunological mechanisms induced by live attenuated and purified inactivated dengue virus vaccines administered in a heterologousprime-boost regimen. Innate and adaptive T and B cell responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach.During the first project year, IRB approvals, inter-institutional agreements, and measurements of dengue virus-specific neutralizing antibody titers and frequencies of cytokine-producing CD4 and CD8 T cells were completed. Flow cytometry of cellular activation and measurement of serum cytokine levels were completed on a subset of subjects. Pilot studies were done for RNA extraction and analysis of B cells and T cell repertoires.

Subject Categories:

  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE