An Integrated System for Automating Artificial Metalloenzyme Evolution
Technical Report,15 Aug 2015,14 Aug 2016
University of Chicago Chicago United States
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Controlling the selectivity of chemical reactions in biological systems, where they could have a transformative impact on manipulating living systems, stand as key challenges in synthetic chemistry. Artificial metalloenzymes ArMs could achieve these goals by combining the reactivity of synthetic metal catalysts and the adaptability and efficiency of enzymes. As part of a research program funded in part by the ARO 62247-LS, we have made several exciting advances in ARM research, including the first demonstration of ARM evolution. As we have developed new techniques to enable these advances, further progress has become limited by the low throughput of the instrumentation available in my laboratory for protein evolution. This not only slows the progress of our proposed research but also saddles students, the creative engines of research, with tedious and repetitious tasks that keep them from tackling higher level problems. This award funded the design and installation of a system for automating most tasks involved with ArM engineering. As expected, this system is widely used by my group and has facilitated and standardized our ArM evolution efforts.