Assessment of Post Remediation Performance of a Biobarrier Oxygen Injection System at a MTBE Contaminated Site
Technical Report,01 Sep 2015,31 Mar 2017
NAVAL FACILITIES ENGINEERING COMMAND PORT HUENEME CA PORT HUENEME United States
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This Environmental Security Technology Certification Program ESTCP-funded project was performed to evaluate the long-term performance of monitored natural attenuation MNA at a site where natural attenuation of methyl tert-butyl ether MTBE is being used as a polishing step following in situ bioremediation. To achieve this goal, two primary objectives were identified -Evaluate the current microbial activity supporting natural attenuation of MTBE using a combination of conventional contaminant concentration data and geochemistry trend analyses and advanced molecular biological tools MBTs, including metaproteomics and metagenomics. -Assess the long-term impact of the biobarrier system on formation permeability. In addition to evaluating data collected using conventional monitoring techniques, this project applied metagenomics and metaproteomics to improve the understanding of long-term impacts of the remedy on biodegradation at the site. Use of these advanced MBTs for quantification and detection of biomarkers, especially deoxyribonucleic acid DNA and peptides protein fragments in environmental samples has been rapidly expanding over the last few decades.
- Water Pollution and Control
- Genetic Engineering and Molecular Biology