Control of Lung Inflammation by Microbiome and Peptidoglycan Recognition Protein
Technical Report,01 Jul 2017,30 Jun 2018
Trustees Of Indiana University Indianapolis United States
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This project is using a mouse model of experimentally-induced asthma to test two hypotheses i that respiratory and intestinal microbiomes control sensitivity to asthma and ii that this microbiome is controlled by antibacterial innate immunity protein, Peptidoglycan Recognition Protein 1 Pglyrp1. Microflora was depleted in mice with antibiotics and pregnant females and their pups were colonized with microfloras from wild-type mice or from Pglyrp1-deficient mice. These pups were then sensitized with house dust mite allergen to induce asthma. Mice colonized with microfloras from wild-type or Pglyrp-deficient mice had similar severity of asthma and lung inflammation, as measured by lung resistance test and extent of infiltration with inflammatory cells. By contrast, germ-free mice completely devoid of microflora, similarly colonized with microbiomes from Pglyrp1-- mice and sensitized, had significantly less severe asthma and lung inflammation than germ-free mice colonized with microbiomes from wild-type mice, as measured by lung resistance test and extent of infiltration with inflammatory cells. These results indicate that microbiome significantly affects sensitivity to asthma and lung inflammation and that microbiome from Pglyrp1-deficient mice reduces allergic inflammatory response in the lungs compared with microbiome from wild-type mice.
- Medicine and Medical Research
- Anatomy and Physiology