Gold:Protein Stoichiometric Analysis of Albumin-Stabilized Gold Nanoclusters
Technical Report,01 Aug 2017,31 Jan 2018
University of Maryland School of Pharmacy Baltimore United States
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The field of bionanomaterials presents an opportunity to leverage the unique functionality inherent within the individual bio and nanocomponents to enable novel hybridized functionalities. Protein-stabilized nanoclusters are a primary example of such a bionano hybrid system, where enhanced coupled functionality has been demonstrated for sensor applications. Currently, however, the factors that control whether a protein component retains its activity are not well understood, but are believed to primarily relate to the location of the cluster stabilization within the host protein. In this work, foundational efforts toward identifying the stabilizing ligands coupled to the nanocluster are performed. Specifically, the goldprotein stoichiometry of abovine serum albumin-nanocluster hybrid is characterized and purification strategies are optimized to allow for subsequent ligand identification routes.
- Physical Chemistry
- Inorganic Chemistry