Sampling Dynamics for Volatile Organic Compounds Using Headspace Solid-Phase Microextraction Arrow for Microbiological Samples (Post Print)
Journal Article - Open Access
Chaminade University of Honolulu Honolulu United States
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Volatile organic compounds VOCs are monitored in numerous fields using several commercially-available sampling options. Sorbent-based sampling techniques, such as solid-phase microextraction SPME, provide pre-concentration and focusing of VOCs prior to gas chromatography mass spectrometry GC-MS analysis. This study investigated the dynamics of SPME Arrow, which exhibits an increased sorbent phase volume and improved durability compared to traditional SPME fibers. A Volatile Reference Mixture VRM and Saturated Alkanes Mix SAM were used to investigate optimal parameters for microbiological VOC profiling in combination with GC-MS analysis. Fiber type, extraction time, desorption time, carryover, and reproducibility were characterized, in addition to a comparison with traditional SPME fibers. The developed method was then applied to longitudinal monitoring of Bacillus subtilis cultures, which represents a ubiquitous microbe in medical, forensic, and agricultural applications. The Carbon Wide RangePolydimethylsiloxane CWRPDMS fiber was found to be optimal for the range of expected VOCs in microbiological profiling.