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Characterization of Clustered CTCs to Eliminate Breast Cancer Metastasis

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Technical Report,01 Jun 2017,31 May 2018

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Case Western Reserve University Cleveland United States

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This program aims to examine the role of platelets and IL-11 in breast cancer tumor cell clustering and metastasis to the organs such as lung. In year 1, we determined that 5 days ofIL-11 treatment to C57BL6, but not SCID or balbc, mice resulted in a 2-3-fold increase in platelet counts. Thus, only IL-11induced thrombocytosis only occurs in C57BL6 mice. In year2 of the program, we then had to create a murine model for metastatic breast cancer inC57BL6 mice. After establishing the institutional protocols to 1 label C57BL6 tumorEO771-LMB cells with luciferase, 2 obtain Case Western Reserve University IACUC approval for these studies, 3 obtain Case Western Reserve University Institutional Biosafety Committee IBC approvals to inject stably transfected tumor cells into mice, and 4 Obtainre-approvals from DOD ACURO of our revised animal protocols, we began experiments using a syngeneic murine mammary cancer tumor in the C57BL6.

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  • Medicine and Medical Research

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