Accession Number:

AD1058598

Title:

Improving Protein Stability and Activity Using Zwitterionic Peptides

Descriptive Note:

Technical Report,01 Jul 2013,31 Mar 2014

Corporate Author:

University of Washington Seattle United States

Personal Author(s):

Report Date:

2017-03-13

Pagination or Media Count:

11.0

Abstract:

This work established the use of polypeptides composed of repeating glutamic acid E and lysine K polyEK for the purpose of increasing protein stability. The addition of polyEK to the primary sequence at the C-terminus of a model beta-lactamase protein and a destabilized TEM-19 demonstrated significant ability in increasing protein stability while maintaining bioactivity. The use of fusion protein products allows for a one-step protocol resulting in a homogeneous product, a significant advantage over polymer-based approaches.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE