Improving Protein Stability and Activity Using Zwitterionic Peptides
Technical Report,01 Jul 2013,31 Mar 2014
University of Washington Seattle United States
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This work established the use of polypeptides composed of repeating glutamic acid E and lysine K polyEK for the purpose of increasing protein stability. The addition of polyEK to the primary sequence at the C-terminus of a model beta-lactamase protein and a destabilized TEM-19 demonstrated significant ability in increasing protein stability while maintaining bioactivity. The use of fusion protein products allows for a one-step protocol resulting in a homogeneous product, a significant advantage over polymer-based approaches.
- Genetic Engineering and Molecular Biology