Accession Number:
AD1058598
Title:
Improving Protein Stability and Activity Using Zwitterionic Peptides
Descriptive Note:
Technical Report,01 Jul 2013,31 Mar 2014
Corporate Author:
University of Washington Seattle United States
Personal Author(s):
Report Date:
2017-03-13
Pagination or Media Count:
11.0
Abstract:
This work established the use of polypeptides composed of repeating glutamic acid E and lysine K polyEK for the purpose of increasing protein stability. The addition of polyEK to the primary sequence at the C-terminus of a model beta-lactamase protein and a destabilized TEM-19 demonstrated significant ability in increasing protein stability while maintaining bioactivity. The use of fusion protein products allows for a one-step protocol resulting in a homogeneous product, a significant advantage over polymer-based approaches.
Descriptors:
Subject Categories:
- Biochemistry
- Genetic Engineering and Molecular Biology