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Pilot Validation of a Hemodilution Technique to Estimate Blood Volume in Vivo

Descriptive Note:

[Technical Report, Final Report]

Corporate Author:

Uniformed Services University

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Purpose Perioperative fluid therapy is used to optimize tissue perfusion. Existing methods to quantify blood volume and estimate intravenous IV fluid requirements are either inaccurate or require specialized equipment and training. This study assessed the utility of mild hemodilution for quantifying blood volume. Design A prospective validation design was used. Blood volume was quantified utilizing two methods in a fixed order radiodilution, then hemodilution as described in D Angelo et al. 2014. Methods After IRB approval and informed consent, blood volume BV estimates were derived for each subject using radiodilution via the Daxor BVA-100, and from pre- and post-bolus hematocrit HcT using methods described in DAngelo, et al. 2014. Hematocrit was assessed via 3 methods Microimpedence via an i-STAT device and the Duke University Medical Center Lab, and total hemoglobin SpHb, Masimo Corporation, Irvine, CA . Sample Thirty-three healthy, male subjects aged 18-35 years. Analysis Bland-Altman plots, the intraclass correlation, and Lins Concordance Coefficient were utilized to compare the gold standard of radiodilution and the proposed method of mild hemodilution for each method of measuring HcT i-STAT, Lab, and SpHb. Findings Mean BV estimates were 5568.6902.2 ml via radiodilution, 8521.23526.5 ml via i-STAT, 16411.013774.5 via Duke lab, and 15882.022371.0 via the Masimo SpHb device. Agreement in estimated BV between methods was low Lins Concordance Coefficient 0.02-0.05. Crystalloid loss to extravasation or renal filtration necessary to account for the observed differences between methods ranged from 181.9 ml to 299.7 ml over 6 minutes.

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

[A, Approved For Public Release]