Accession Number:

AD1058206

Title:

A New Paradigm for Ovarian Sex Cord-Stromal Tumor Development

Descriptive Note:

Technical Report,15 Apr 2015,14 Apr 2018

Corporate Author:

Texas A and M Agrilife Research College Station United States

Personal Author(s):

• Li,Qinglei

Report Date:

2018-07-01

Pagination or Media Count:

16.0

Abstract:

Transforming growth factor beta TGFB signaling regulates fundamental reproductive events via TGFB receptor complexes TGFBR1TGFBR2 and downstream SMAD proteins. To determine potential effect of overactivation of TGFB signaling in distinct cellular compartments, we generated a mouse model containing a constitutively active TGFBR1 using growth differentiation factor 9 Gdf9-Cre termed TGFBR1-gCA. We showed that sustained activation of TGFBR1 disrupts folliculogenesis via affecting ovarian reserve and follicle growthdevelopment. Ovarian tumor tissues from TGFBR1-gCA mice were positive for granulosa cell markers. RNA-Seq analysis using ovarian RNA from TGFBR1-gCA mice and controls identified a number of genes associated with folliculogenesis, oogenesis, proliferation, and differentiation. Histological and molecular analyses provided evidence of overactivation of TGFB signaling in ovarian granulosa cell compartment. The mouse model may be further exploited to define the cellular and molecular mechanisms of TGFBactivin downstream signaling in granulosa cell tumor development.

Descriptors:

• GROWTH FACTORS
• neoplasms
• pathogenesis
• RNA SEQUENCE ANALYSIS
• ribonucleic acids
• proteins
• OVARIES
• histology

Subject Categories:

• Medicine and Medical Research
• Genetic Engineering and Molecular Biology
• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE