Accession Number:

AD1058100

Title:

Assessment of Post Remediation Performance of a Biobarrier Oxygen Injection System at a Methyl Tert-Butyl Ether (MTBE)-Contaminated Site, Marine Corps Base Camp Pendleton San Diego, California

Descriptive Note:

Technical Report,01 Sep 2015,31 Mar 2017

Corporate Author:

NAVAL FACILITIES ENGINEERING COMMAND PORT HUENEME CA PORT HUENEME

Report Date:

2017-05-01

Pagination or Media Count:

284.0

Abstract:

This Environmental Security Technology Certification Program ESTCP-funded project was performed to evaluate the long-term performance of monitored natural attenuation MNA at a site where natural attenuation of methyl tert-butyl ether MTBE is being used as a polishing step following in situ bioremediation. To achieve this goal, two primary objectives were identified Evaluate the current microbial activity supporting natural attenuation of MTBE using a combination of conventional contaminant concentration data and geochemistry trend analyses and advanced molecular biological tools MBTs, including metaproteomics and metagenomics. Assess the long-term impact of the biobarrier system on formation permeability. In addition to evaluating data collected using conventional monitoring techniques, this project applied metagenomics and metaproteomics to improve the understanding of long-term impacts of the remedy on biodegradation at the site. Use of these advanced MBTs for quantification and detection of biomarkers, especially deoxyribonucleic acid DNA and peptides protein fragments in environmental samples has been rapidly expanding over the last few decades.

Subject Categories:

  • Solid Wastes and Pollution and Control
  • Solid Wastes and Pollution and Control

Distribution Statement:

APPROVED FOR PUBLIC RELEASE