Accession Number:

AD1057905

Title:

Genome Sequencing to Enable a Model Salamander for Tissue Regeneration Research

Descriptive Note:

Technical Report,01 Oct 2011,31 Oct 2014

Corporate Author:

University of Kentucky Lexington United States

Personal Author(s):

Report Date:

2016-05-06

Pagination or Media Count:

13.0

Abstract:

Salamanders are important vertebrate model organisms in regenerative medicine. In this application, we proposed to enhance research resources for the Mexican axolotl Ambystoma mexicanum by innovating an approach to sequence their large 32,000,000,000 base pair genome. We successfully developed methods to isolate and sequence whole axolotl chromosomes, and published a manuscript in a leading journal that describes our approach and initial characterization of the axolotl genome. Thus, we accomplished the objectives of our project, laying the ground work for developing a full axolotl genome assembly.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE