Genome Sequencing to Enable a Model Salamander for Tissue Regeneration Research
Technical Report,01 Oct 2011,31 Oct 2014
University of Kentucky Lexington United States
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Salamanders are important vertebrate model organisms in regenerative medicine. In this application, we proposed to enhance research resources for the Mexican axolotl Ambystoma mexicanum by innovating an approach to sequence their large 32,000,000,000 base pair genome. We successfully developed methods to isolate and sequence whole axolotl chromosomes, and published a manuscript in a leading journal that describes our approach and initial characterization of the axolotl genome. Thus, we accomplished the objectives of our project, laying the ground work for developing a full axolotl genome assembly.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research