Accession Number:

AD1057859

Title:

A Stress-Induced Bias in the Reading of the Genetic Code in Escherichia coli

Descriptive Note:

Journal Article - Open Access

Corporate Author:

The Hebrew University-Hadassah Medical School Jerusalem Israel

Report Date:

2016-11-15

Pagination or Media Count:

11.0

Abstract:

Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin TA system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. Thereby, under stress, the induced MazF generates a stress-induced translation machinery STM, composed of MazF-processed mRNAs and selective ribosomes that specifically translate the processed mRNAs. Here, we further characterized the STM system, finding that MazF cleaves only ACA sites located in the open reading frames of processed mRNAs, while out-of-frame ACAs are resistant. This in-frame ACA cleavage of MazF seems to depend on MazF binding to an extracellular-death-factor EDF-like element in ribosomal protein bS1 bacterial S1, apparently causing MazF to be part of STM ribosomes. Furthermore, due to the in-frame MazF cleavage of ACAs under stress, a bias occurs in the reading of the genetic code causing the amino acid threonine to be encoded only by its synonym codon ACC, ACU, or ACG, instead of by ACA.

Subject Categories:

  • Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE