Development of a Non-Human Primate (Rhesus Macaque) Model of Upper Limb Vascularized Composite Allotransplants
Technical Report,01 Sep 2016,30 Mar 2018
Naval Medical Research Unit San Antonio Fort Sam Houston United States
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Background Traumatic limb amputations represent a substantial challenge facing many civilian and combat wounded. Despite advancements in VCA research to date, a tissue preservation strategy that successfully mitigates ischemia reperfusion injury, preserves graft functionality, and provides long-term graft survival remains imperfect. Objective To develop a non-human primate NHP model of upper limb vascularized composite allotransplant, that allow for evaluation of tissue preservation and reperfusion strategies. Materials and methods Upper extremities were procured from male rhesus macaques n17. Cold storage preservation with University of Wisconsin solution for 12 hours at 4 degrees Celsius was performed, followed by reperfusion of the limbs using extracorporeal membrane oxygenation and a pulsatile perfusion pump with warm autologous whole blood. Results The results shown here are gathered from n5 extremities. Significant changes 1 compared to BSLN lactate and potassium were significantly increased at R60, 2 Base deficit significantly decreased at R0 and R60, 3 Cytokines IL-8 and sCD40-L were significantly increased at R60. Histopathological analysis improved after reperfusion. Conclusions This protocol establishes a baseline for a standardized NHP limb preservation and is the first step in developing an ex vivo pre-transplantation assessment tool providing statistical analysis of projected graft function and survival.
- Medicine and Medical Research