Yeast Surface Display Approaches for Engineering Stabilized Viral Fusion Protein Subunit Vaccines
Technical Report,15 Apr 2017,14 Apr 2018
Leland Stanford Junior University Stanford United States
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This research proposal focuses on the development of a novel library screening approach to engineering highly stabilized subunit vaccine candidates for major pathogens within the paramyxovirus family. The research addresses the PRMRP topic areas related to vaccine development for infectious pathogens as well as addressing the topic concerning emergent viruses. The paramyxoviruses encompass many pathogens that cause disease in humans and this proposal focuses on four viruses that fall into two subclasses within the broader family, respiratory syncytial virus RSV, human metapneumovirus HMPV, Nipah virus NiV and HendraVirus HeV. In the past year we have been exploring different approaches to expressing F protein trimers at the surface of yeast and have developed two novel strategies that utilize self-cleaving 2A peptides and reporter fluorescent proteins as our foundational constructs for F library generation and selection.
- Medicine and Medical Research