Accession Number:

AD1053574

Title:

Toxin MqsR Cleaves Single-Stranded mRNA with Various 5' Ends

Descriptive Note:

Journal Article - Open Access

Corporate Author:

Department of Chemical Engineering, Pennsylvania State University University Park United States

Report Date:

2016-02-05

Pagination or Media Count:

8.0

Abstract:

Toxinantitoxin TA systems are the means by which bacterial cells become persistent that is, those cells that are tolerant to multiple environmental stresses such as antibiotics by becoming metabolically dormant. These persister cells are responsible for recalcitrant infections. Once toxins are activated by the inactivation of antitoxins e.g., stress-triggered Lon degradation of the antitoxin, many toxins reduce metabolism by inhibiting translation e.g., cleaving mRNA, reducing ATP. The MqsRMqsA TA system of Escherichia coli cleaves mRNA to help the cell withstand oxidative and bile acid stress. Here, we investigated the role of secondary structure and 5 mRNA processing on MqsR degradation of mRNA and found that MqsR cleaves only single-stranded RNA at 5-GCUsites and that MqsR is equally active against RNA with 5-triphosphate,5-monophosphate, and 5-hydroxylgroups.

Subject Categories:

  • Toxicology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE