Accession Number:

AD1053311

Title:

Custom Super-Resolution Microscope for the Structural Analysis of Nanostructures

Descriptive Note:

Technical Report,15 Jul 2016,28 Feb 2018

Corporate Author:

HARVARD COLL CAMBRIDGE MA CAMBRIDGE United States

Personal Author(s):

Report Date:

2018-05-29

Pagination or Media Count:

7.0

Abstract:

Super-resolution optical microscopy is a powerful method for visualizing nanostructures and biomolecules in their native, in situ environments. We have constructed a custom super-resolution microscope optimized for the high-throughput, super-resolution structural mapping of nanostructures and biological targets. Our microscope features a large field of view and custom optics that facilitate 3D imaging and enhanced contrast in noisy samples while providing a simple and user-friendly interface. Since recently completing the construction of this instrument, we have begun to optimize its 3D imaging capability, and it has been used to help develop a novel programmable molecular tool for the amplification of fluorescent signals in fixed cells and tissues. In parallel, we have augmented an existing microscope with a high-resolution scanning confocal modality, improving our imaging throughput and opening up high-throughput super-resolution imaging to a broader group of researchers. Collectively, this instrumentation will play a critical role in technology development and materials characterization for the greater Wyss Institute and Harvard Systems Biology community.

Subject Categories:

  • Optics
  • Electrical and Electronic Equipment

Distribution Statement:

APPROVED FOR PUBLIC RELEASE