Custom Super-Resolution Microscope for the Structural Analysis of Nanostructures
Technical Report,15 Jul 2016,28 Feb 2018
HARVARD COLL CAMBRIDGE MA CAMBRIDGE United States
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Super-resolution optical microscopy is a powerful method for visualizing nanostructures and biomolecules in their native, in situ environments. We have constructed a custom super-resolution microscope optimized for the high-throughput, super-resolution structural mapping of nanostructures and biological targets. Our microscope features a large field of view and custom optics that facilitate 3D imaging and enhanced contrast in noisy samples while providing a simple and user-friendly interface. Since recently completing the construction of this instrument, we have begun to optimize its 3D imaging capability, and it has been used to help develop a novel programmable molecular tool for the amplification of fluorescent signals in fixed cells and tissues. In parallel, we have augmented an existing microscope with a high-resolution scanning confocal modality, improving our imaging throughput and opening up high-throughput super-resolution imaging to a broader group of researchers. Collectively, this instrumentation will play a critical role in technology development and materials characterization for the greater Wyss Institute and Harvard Systems Biology community.
- Electrical and Electronic Equipment