Optimized Liposomal Dexamethasone Therapy Improves Functional Outcome of Post-Traumatic Skeletal Muscle and Neuromuscular Junction
Technical Report,01 Mar 2017,28 Feb 2018
University of Nebraska Medical Center Omaha United States
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In this fiscal year 03012017 to 02282018, we performed our research experiments in four groups of mice including sham, tourniquet-induced IRsaline, tourniquet-induced IRlipo-Dex, and tourniquet-induced IRDex groups. These experiments tested the protective effects of lipo-Dex and Dex on skeletal muscle morphology and function in mice with tourniquet-induced IR. Lipo-Dex is liposome-encapsulated Dex. Our study found that lipo-Dex was mainly retained in IR-injured skeletal muscles after 24-hour ischemiareperfusion IR, lasted about 1 week, and then disappeared. Unilateral left hindlimb ischemia was induced in IR mice by placing an orthodontic rubber band ORB at the hip joint through use of a McGivney hemorrhoidal ligator. After 3 hours of ischemia, the ORB tourniquet was released to begin reperfusion for different periods. Treatment with Dex 1mgkgday, i.p. injection for 1 wk or lipo-Dex 7mgkg, one-time i.v., equivalent to 1 mgkgday of Dex for 1 wk will begin at the beginning of reperfusion on the day of IR induction. Our data demonstrated that treatment of lipo-Dex or Dex suppresses IR-induced inflammation and promotes recovery of skeletal muscle morphology and function from tourniquet-induced IR injury.
- Medicine and Medical Research
- Anatomy and Physiology