Systems Biology of Immune Response to Live and Inactivated Dengue Virus Vaccines
Technical Report,01 Sep 2016,31 Aug 2017
The Geneva Foundation Tacoma United States
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The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive T and B cell responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the first project year, IRB approvals, inter-institutional agreements, and measurements of dengue virus-specific neutralizing antibody titers and frequencies of cytokine-producing CD4 and CD8 T cells were completed. Flow cytometry of cellular activation and measurement of serum cytokine levels were completed on a subset of subjects. Pilot studies were done for RNA extraction and analysis of B cells and T cell repertoires.
- Anatomy and Physiology