Accession Number:

AD1050791

Title:

Probing Androgen Receptor Signaling in Circulating Tumor Cells in Prostate Cancer

Descriptive Note:

Technical Report,01 Jul 2012,30 Jun 2017

Corporate Author:

Massachusetts General Hospital Boston United States

Personal Author(s):

Report Date:

2017-10-01

Pagination or Media Count:

132.0

Abstract:

This Physician Research Training Award supports a mentored training program integrated with a research project focused on the study of circulating tumor cells CTCs in prostate cancer patients using a microfluidic device CTC-chip to isolate CTCs from peripheral blood samples. During this award, we developed a quantitative immunofluorescence assay to measure AR activity in CTCs in patients with metastatic prostate cancer receiving treatment with hormonal therapy. We also developed methods for single CTC RNA-seq and digital gene expression profiling, and applied these techniques to single prostate CTCs. We analyzed single cell transcriptomes to reveal the heterogeneity of CTCs, and to identify key genes and molecular pathways enriched in CTCs that may contribute to treatment resistance in prostate cancer, including AR splice variants and the non-canonical Wnt pathway. Finally, this Award provided valuable protection of time for research and mentored training of the PI, and was essential in enabling his development towards a productive independent career in translational prostate cancer research.

Subject Categories:

  • Medicine and Medical Research
  • Medical Facilities, Equipment and Supplies
  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE