Identification of Immunogenic Targets for Lung Cancer Vaccines
Technical Report,01 Sep 2016,31 Aug 2017
University of Washington Seattle United States
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The goal of this project is to develop a vaccine for lung cancer prevention in current or past smokers by identifying immunogenic proteins in lung cancer that are able to induce a potent inflammatory Th1 response. Proteins overexpressed in tumors can be immunogenic. We have used quantitative proteomic analysis to identify proteins overexpressed in non-small cell lung cancer cell lines compared with normal lung epithelial cells. Candidate antigens were investigated by siRNA screening to identify those genes with a function in maintaining cell tumor growth. If a gene is required for tumor cell proliferation, knocking down the gene by siRNA should decrease cell survival and proliferation. We identified 14 candidates that are overexpressed in lung cancer and necessary for tumor cell survival. We have prioritized those proteins which have been previously described to play a role in lung cancer invasion, proliferation, metastasis or survival. We selected 5 of those proteins to move forward. We have done in silico screening for HLA-DR epitope prediction and we have been able to identify epitopes with a high probability of been presented by multiples HLA-DR alleles. Therefore, during this period of the award, we have identified possible antigens and epitopes to develop a multi-antigen vaccine to prevent lung cancer.
- Medicine and Medical Research