Accession Number:

AD1049013

Title:

Using a Novel Transgenic Mouse Model to Study c-Myc Oncogenic Pathway in Castration Resistance and Chemoresistance of Prostate Cancer

Descriptive Note:

Technical Report,15 Sep 2013,14 Sep 2017

Corporate Author:

Baylor College of Medicine Houston United States

Personal Author(s):

• Yang, Feng

Report Date:

2017-12-01

Pagination or Media Count:

15.0

Abstract:

We previously made a PB-Cre4Ai-Myc model for Cre-induced and androgen-independent expression of c-Myc and Luc2 in prostate. This is designed for concisely studying castration response and CRPC. However, most mice never developed significant tumors. Here, we showed that ablation of p53 in this model led to rapid growth of prostate tumors expected based on our hypothesis but with lethal epididymis tumors unexpected. To solve this problem, we performed early castration together with testosterone pellet implantations in these mice. The mice were later divided into two groups, the control continued receiving pellet implantations whereas the castration group underwent a pellet removal procedure. We observed castration-response as evidenced by greatly reduced BLI signal and a lack of AR nuclear localization in the castrated tumors. However, we did not observe significant difference in overall survival and there were large seminal vesicle tumors associated with most prostate tumors in this model. These made it difficult to concisely assess prostate tumor response to castration. We have designed and will use alternative approaches to address this problem in the near future.

Descriptors:

• prostate cancer
• resistance (biology)
• neoplasms
• chemotherapy

Subject Categories:

• Genetic Engineering and Molecular Biology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE