Accelerating Coagulation in Traumatic Injuries Using Inorganic Polyphosphate-Coated Silica Nanoparticles in a Swine (Sus scrofa) Model
Technical Report,21 Jul 2016,15 Mar 2018
DAVID GRANT USAF MEDICAL CENTER TRAVIS AFB CA TRAVIS AFB
Pagination or Media Count:
Objective To determine if platelet-like nanoparticles PLNP will decrease blood loss in a swine in a swine model. Methods Pigs were anesthetized and instrumented and randomized to receive either PLNP or normal saline. After 10 minutes, the left lateral liver lobe was sharply dissected with trauma shears. Pressure was applied using hand pressure and 3 lap pads. Three minutes later pressure was released and a suction drain placed. The study ended after 1 hour. The cut liver lobe was clamped and the animal was euthanized. Lap sponges and all free blood and clots were carefully removed and weighed.Results There were no significant differences between the groups in any baseline characteristics, preoperative blood loss, or liver injury surface area. Pigs receiving PLNP lost 13.5 mL of blood per kg vs. controls who averaged 22.8 mLkg, but the difference was not significant. There were no significant differences between groups in physiologic measures, lab studies, or histopathology evaluation at the end of the study. Conclusion Based on these results, we conclude there was no significant difference in blood loss between controls and pigs receiving PLNP. There was no evidence of thrombus formation in any of the tissues examined.
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