Acceleration of Regeneration of Large-Gap Peripheral Nerve Injuries Using Acellular Nerve Allografts Plus Amniotic Fluid Derived Stem Cells (AFS)
Technical Report,01 Sep 2016,31 Aug 2017
Wake Forest University Health Science Winston-Salem United States
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Major accomplishments this year include the use of AFS seeded A cellular Nerve Allografts ANA to repair critical size nerve defects 1.5 cm in rats. Functional recovery was monitored longitudinally using digital video gait analysis as well as electrophysiologic and histologic outcomes. The results demonstrated that the AFS seeded ANA used for nerve repair resulted in an improved functional outcome for the rats compared to ANA alone and were equivalent to those repaired using nerve autograft, the current gold standard for tension-free repair of transected peripheral nerves. Axon counts and neuromuscular junction morphology were equivalent between the AFS seeded ANA. Additional studies investigated the use of post-partum a cellular materials to promote Schwann cell proliferation as well as renewed investigations into decellularizationoxidation of nerves. The coming year will utilize these techniques for repairing large-gap 6 cm nerve injuries in non-human primates. This pre-clinical model represents a more translational model of peripheral nerve injury and repair. In addition, preservation of neuromuscular junctions using beta 2 agonists will be studied. IACUC and ACURO approvals for these studies were renewed.
- Medicine and Medical Research