Functions of Tenascin-C and Integrin alpha9beta1 in Mediating Prostate Cancer Bone Metastasis
Technical Report,30 Sep 2016,29 Sep 2017
Baylor College of Medicine Houston United States
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The purpose of this work is to dissect mechanisms responsible for interactions between integrin a9b1 and tenascin-C that are fundamental in prostate cancer metastasis to bone. Task1 is to address the role of a9b1 using gene knockdown followed by assessment of pathway activation downstream of a9b1 and their effects on prostate cancer biology. Following someinitial difficulties regarding cell viability, Substask 1 of Task 1 is nearly completed. We have generated a line of VCaP cells with inducible knockdown of alpha9 integrin. Studies arein progress to generate additional engineered cell lines for verification and we plan to also generate stable knockout cell lines using CRISPRCas 9 gene editing technology. Subtask 2studies have been initiated. Substask 1 of Major Task 2 is also completed. Once engineered cell lines are validated and tested, we will initiate the remaining Subtask 3 of Major Task 1as well as Task 2 studies. This work will advance the field by providing mechanistic data regarding the role of alpha 9 and tenascin-C in the biology of prostate cancer bone metastasis.
- Medicine and Medical Research