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Evaluation of Novel Antimicrobial Peptides as Topical Anti-Infectives with Broad-Spectrum Activity against Combat-Related Bacterial and Fungal Wound Infections

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Technical Report,30 Sep 2016,29 Sep 2017

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Riptide Bioscience, Inc Vallejo United States

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Ballistic wound infection has become the greatest threat to the life and recovery of the combat casualty who survives the immediate trauma of the insult. Multidrug resistance and generation of recalcitrant biofilm are major obstacles in treating wounds. Antimicrobial peptides AMPs, also known as host defense peptides, are evolutionarily highly conserved components of the innate immune system that provide the first line of defense against invading pathogens in all multicellular organisms. Designed antimicrobial peptides dAMPs are synthesized peptides that have been rationally designed based on sequences found in naturally occurring AMPs. dAMPs are amphipathic cationic peptides with the ability to kill microbes by disrupting their membrane function. This mode of action rapidly kills antibiotic resistant microbes, even in biofilm. Bacteria are thus less likely to develop resistance to AMPs. Riptide Bioscience has synthesized thirty-one novel dAMPs in four iterative rounds. The dAMPs were evaluated for their antimicrobial potency against 11 strains of gram positive and gram negative bacteria and 7 strains of fungi. Standard MIC Minimum Inhibitory Concentration assays were conducted according to CLSI guidelines. Cytotoxicity was determined using L929 fibroblasts into which the luciferase gene had been transfected. Time-kill assays used P. aeruginosa Xen5 and S. aureus Xen36 cultures.

Subject Categories:

  • Medicine and Medical Research
  • Biochemistry
  • Microbiology

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