Accession Number:

AD1048149

Title:

Mouse and Human Models for Investigating Influences of Tau on Progression of Alzheimer's Disease Following Traumatic Neuronal Injury

Descriptive Note:

Technical Report,15 Sep 2016,14 Sep 2017

Corporate Author:

University of California, San Diego La Jolla United States

Personal Author(s):

Report Date:

2017-10-01

Pagination or Media Count:

10.0

Abstract:

We have completed the design, fabrication, and validation of a new biomedical device to impose moderate mechanical loads on cultured stem-cell derived neuronal and glial cells. Using this device, we assessed morphological changes, beta amyloid production, and tauphosphorylation i.e., multiple Alzheimers-associated outcomes following rapid stretch of iPSC-derived neurons. Results suggest that neurites oriented in the direction of substrate stretch were non-elastically deformed, the cytoskeleton was reorganized, a myloid precursor protein transport was perturbed, amyloid production was increased, and tau phosphorylation unchanged following a single bout of mechanical loading. Multiple bouts of mechanical loading amplified amyloid and tau phenotypes, suggesting a dependence of these Alzheimers associated outcomes to injury dose or severity.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE