Mouse and Human Models for Investigating Influences of Tau on Progression of Alzheimer's Disease Following Traumatic Neuronal Injury
Technical Report,15 Sep 2016,14 Sep 2017
University of California, San Diego La Jolla United States
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We have completed the design, fabrication, and validation of a new biomedical device to impose moderate mechanical loads on cultured stem-cell derived neuronal and glial cells. Using this device, we assessed morphological changes, beta amyloid production, and tauphosphorylation i.e., multiple Alzheimers-associated outcomes following rapid stretch of iPSC-derived neurons. Results suggest that neurites oriented in the direction of substrate stretch were non-elastically deformed, the cytoskeleton was reorganized, a myloid precursor protein transport was perturbed, amyloid production was increased, and tau phosphorylation unchanged following a single bout of mechanical loading. Multiple bouts of mechanical loading amplified amyloid and tau phenotypes, suggesting a dependence of these Alzheimers associated outcomes to injury dose or severity.
- Medicine and Medical Research