Joint Distraction Treatments of Intra-Articular Fracture-Induced Posttraumatic Osteoarthritis in a Large Animal Model
Technical Report,30 Sep 2016,29 Sep 2017
The University of Iowa Iowa City United States
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Post-traumatic osteoarthritis PTOA is a common complication after an intra-articular fracture IAF in both combat and civilian settings. The broad goal of this expansion award is to determine if joint distraction can prevent the development of PTOA after an IAF. During this second project year we continued analysis of the specimens generated during Project Year 1, developing and implementing new routines for confocal microscopy image analysis, contrast-enhanced micro CT analysis, and kinematic analysis. We also performed Western blots to analyze the synovial fluid harvested from the experimental joints. Results from this work corroborate our macroscopic finding that delays in fracture fixation do have a negative effect on cartilage, and immediate joint distraction did not have a therapeutic benefit. Our research team suffered a personal and professional loss with the death of our histologist which significantly delayed final histological analysis specimens from this first series. During this second project year we also completed all surgeries on the remaining animals in Specific Aim 1. Groups comparing immediate and delayed fracture fixation were completed in the spring and the remaining distracted group just prior to the end of the Project Year. Joint distraction was performed through the articulated distraction device that was developed this Year for use in the upcoming Aim 2 studies.
- Medicine and Medical Research