Therapeutic Evaluation of Mesenchymal Stem Cells in Chronic Gut Inflammation
Technical Report,05 Aug 2011,12 Aug 2016
Texas Tech University Health Sciences Center Lubbock United States
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The overall objective of this proposal is to evaluate the therapeutic efficacy of human, bone marrow-derived mesenchymal stem cells hMSCs in a mouse model of inflammatory bowel disease IBD. As documented in our previous Progress Reports, we encountered a number of major problems following relocation to our current institution that significantly delayed the initiation of the project. By the far the most perplexing and time-consuming problem was the loss of disease phenotype of our mouse model of IBD that we had used for several years during my tenure at LSU Health Sciences Center LSUHSC. We hypothesized that the loss of phenotype was due to significant differences in the microbial composition of mice housed at the two animal facilities. Thus began, a 3 year process to re-derive our original mouse model of chronic colitis. To do this, we undertook a systematic study to quantify and compare the intestinal microbiota within disease susceptible mice housed at LSUSHC vs. disease-resistant mice that were housed at Texas Tech University HSC.
- Medicine and Medical Research