Small Molecule Protection of Bone Marrow Hematopoietic Stem Cells
Technical Report,30 Sep 2014,29 Sep 2015
University of Washington Seattle United States
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During the above project period we have demonstrated the following progress on Aims 1 and 2, and have keyed the following results to specific major tasks under each Aim 1. mitotic expansion of human mobilized peripheral blood CD34 positive hematopoietic stem cells HSCs in culture in defined medium supplemented with hematopoietic growth factors and small molecule inhibitors of differentiation 2. suppression of differentiation of CD34 positive CD38 negative CD90 positive during mitotic expansion in culture by addition of the small molecule inhibitors SR-1 or UM-171, alone or in concert 3. dose-dependent killing of CD34 positive CD38 negative CD90 positive HSCs by the addition of formaldehyde 4. development of sensitive and specific mass spec assays for DNA adducts and DNA-protein crosslinks DPCs generated by formaldehyde in human cells.
- Medicine and Medical Research