GENOMIC DIVERSITY AND THE MICROENVIRONMENT AS DRIVERS OF PROGRESSION IN DCIS
Technical Report,30 Sep 2016,29 Sep 2017
Duke University Durham United States
Pagination or Media Count:
The project is designed to test whether genetic andor tumor environmental heterogeneity is a driving force in progression of breast DCIS. Our project, a collaboration between Duke and ASU, has made substantial progress on all 4 aims and we met our 36 month milestones. Primary achievements for 36 months are 1 Continued Case and control identification 45 Pure DCIS and 36 adjacent DCIS with invasion through extensive database and searching at Duke 2 Deep and comprehensive full exome sequencing for 32 cases from 30-160ng of DNA isolated from archival FFPE specimens, 3 Comparison of analytic methods to characterize somatic mutations from this full exome sequencing, 4 Application of sequencing data for copy number assessment 5 Development of dual immune-staining on DCIS lesions using 7 pairs of antibodies, 6 Imaging analysis of these stains, including quantitative analysis, 7 Identification of upstaged DCIS cases for the radiology aim, 8 Development of image analysis methods for digital mammograms, 9 Validation Aim 4 approval of the Duke IRBTBCRC038 protocol at 12 sites, including DOD approval to initiate collection of DCIS that either did or did not progress to invasive cancer, 10 Full integration of team members over the past year via frequent conferencing, face to face meetings, and constant communication. This multi-disciplinary progress puts our group into an ideal position to fully implement the aims of the project and reach our year 4 goals.
- Medicine and Medical Research