Potential Application of Viral Empty Capsids for the Treatment of Acute Lung Injury/Acute Respiratory Distress Syndrome
Technical Report,01 Jul 2015,31 Dec 2016
Hebrew University of Jerusalem Jerusalem Israel
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The goal of this project has been to test the hypothesis that recombinant empty capsids of SV40, Virus Like Particles VLPs, may attenuate ARDS, increasing survival and recovery from this severe clinical condition. We used the rat 2CLP cecal ligation and two punctures model for sepsis-induced ARDS. The VLPs, or vehicle saline control, were administered in 3 equal portions total dose 0.3 mgkg VLPs, in saline for 3 days prior to the 2CLP operation. 2CLP was performed 24 hours later. While 100 the untreated rats died in 4 days, 75 of the VLP-treated ones survived. During the first 48 hours the treated rats presented similar symptoms and weight loss to the untreated ones. However afterwards their deterioration decelerated and they proceeded to full recovery. Lung pathology was normal in both treated and untreated rats sacrificed 24 hours after the 2CLP operation.
- Medicine and Medical Research