Autologous Hematopoietic Stem Cell Transplantation to Prevent Antibody Mediated Rejection After Vascularized Composite Allotransplantation
Technical Report,15 Sep 2016,14 Sep 2017
Johns Hopkins University Baltimore United States
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The investigators successfully performed initial control experiments in unsensitized animals applying the proposed induction and maintenance immunosuppressive regimen to assess its safety and feasibility for maintaining VCA allograft survival in this model. Successful VCA i.e. swine heterotopic hind limb transplantation was performed across a full SLA mismatch from a CC donor to an AD recipient animal. The animal was treated with non-myeloablative total body irradiation and thymic irradiation two days prior to transplantation and with continues calcineurin inhibitor-based immunosuppressive maintenance therapy using tacrolimus at target trough levels of 10-20 ngml for 30 days.
- Medicine and Medical Research