The Role of Tissue-Resident Donor T Cells in Rejection of Clinical Face Transplants
Technical Report,30 Sep 2016,29 Sep 2017
Brigham and Women's Hospital Boston United States
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Unlike solid organ transplants, face transplants have a unique immunological characteristic the presence of skin, which contains approximately 1 million T cellcm2. A full face transplant is 600-700 cm2 in size and therefore, contains approximately 600-700 million donor T cells. The long-term persistence of donor T cells within facial allografts and the role of donor vs. recipient T cells in face transplant rejection are two critical issues that have never been studied. We plan to i quantify the number and diversity of donor vs. recipient T cells within face transplants over time using cutting edge high throughput TCR sequencing HTS, ii identify pathogenic T cell clones implicated in rejection, iii identify if these pathogenic T cell clones are of donor vs. recipient, and iv determine if these pathogenic T cell clones are measurable in blood during episodes of rejection and could therefore serve as an earl and personalized biomarker of rejection.
- Medicine and Medical Research
- Anatomy and Physiology