Smartphone Enabled Point-of-Care Diagnostics for Operationally Significant Pathogens
Technical Report,28 Sep 2016,27 Sep 2017
Duke University Durham United States
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We developed a novel high-throughput screen in microarray format that identifies optimal pairing of antibody pairs, which led to successful identification of optimal antibody reagents for Ebola having excellent sensitivity. We have identified 24 highly promising clones for antibody reagents used for detecting Salmonella. We successfully developed an approach for self-calibrating assay chips that correlate fluorescence intensities to the true known concentration of spiked Ag in serum. We have also identified an optimal strategy for labeling our reagents that is well-suited for our portable assay reader. Furthermore, we have developed 3 redundant designs for our ruggedized, portable reader in case of primary device failure. For assay cassettes, we have devised a simple, low-cost chip that encases all reagents and test components in a user-friendly format. Finally, we devised a development strategy for a smartphone app that automates assay readout.
- Medicine and Medical Research