Inactivation of West Nile Virus in Serum with Heat, Ionic Detergent, and Reducing Agent for Proteomic Applications (Open Access Publisher's Version)
Journal Article - Open Access
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD FORT DETRICK United States
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Research involving biosafety level 3 pathogens such as West Nile virus WNV is often limited by the limited space and technical constraints of these environments. To conduct complex analytical studies outside of high containment, robust and reliable inactivation methods are needed that maintain compatibility with downstream assays. Here we report the inactivation of WNV in spiked serum samples using a commercially available SDSPAGE sample buffer for proteomic studies. Using this method, we demonstrate its utility by identification proteins differentially expressed in the serum of mice experimentally infected with WNV.
- Medicine and Medical Research