Alkaline Phosphatase for the Prevention of Intestinal and Renal Injury in a Rat Model of Cardiopulmonary Bypass with Deep Hypothermic Circulatory Arrest
Technical Report,15 Aug 2016,14 Aug 2017
University of Colorado Aurora United States
Pagination or Media Count:
Purpose To determine if alkaline phosphatase decreases intestinal and renal injury in a piglet model of cardiopulmonary bypass with DHCA, mediated in part through adenosine signaling pathways. Scope Evaluate histologic primary outcome, physiologic, and biomarker evidence of intestinal and kidney injury in this model with administration of escalating doses of bovine intestinal alkaline phosphatase or specific inhibitors of native alkaline phosphatase. Assess the role of downstream adenosine as a mediator of alkaline phosphatase effect. Major Findings For the first reporting period, we had successfully established the model, demonstrating appropriate levels of physiologic disturbance hemodynamic instability and lactic acidosis. Systemic interventions are nearly complete, at which time the initial analysis of the primary outcome will be performed for these groups.
- Medicine and Medical Research