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Evaluating the Genetic, Hormonal, and Exogenous Factors Affecting Somatic Copy Number Variation in Breast Cancer

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Technical Report,30 Sep 2015,29 Sep 2016

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Brigham and Womens Hospital Boston United States

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Here, we outline the progress we have made in the first year of the funding period. Our specific aims are to 1 quantitatively assess genomic instability in different mammary epithelial populations in vivo and in vitro, 2 determine how mutations in heritable breast cancer genes affect genomic stability in different mammary epithelial populations in vivo and in vitro and 3 determine the influence of estrogen and ionizing radiation on genomic stability in the mammary epithelium. Year 1 tasks in our Statement of Work exclusively relate to aim 1. We have made significant progress in sub aim 1a, substantially improving the design of our proposed transgenic animal, the deletion reporter mouse, and are finalizing cloning of necessary components. We expect to submit embryonic stem cells to the transgenic facility within the next few months. Furthermore, subaim 1b is nearing completion. As planned, we have successfully developed and validated an in vitro deletion reporter, which can now be used to assess the influence of genetic and exogenous factors on deletion rate in mammary epithelial cells.

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  • Medicine and Medical Research

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