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Nanotechnology-Based Detection of Novel microRNAs for Early Diagnosis of Prostate Cancer

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Technical Report,15 Jul 2016,14 Jul 2017

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University of Nebraska Omaha United States

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Prostate cancer PCa is most commonly diagnosed and second leading cause of death in the US men, representing 8 of all male cancer deaths. Current gold standard for clinical detection of PCa consist of serum PSA test together with digital rectal examination DRE. However, PSA screening predicted lower sensitivity 21 for PCa, while DRE is mostly linked with the patient discomfort due to painful procedure with a risk of slight bleeding and more importantly, poor sensitivity and specificity. Considering such limitations, there is an urgent need for a novel, non-invasive serum-based biomarker in conjunction with high-sensitive technique to differentiate indolent vs. aggressive prostate cancer PCa. Emerging evidence suggests that several micro RNAs miRs are differentially expressed in tissues and sera of PCa patients and can potentially serve as biomarkers for disease progression and aggressiveness. However, quantification of ultra-low levels of serum miRNAs remains difficult with conventional quantification methods. Hence, present study was aimed at developing, optimizing and testing a novel DNA-gold nanoprobe DNA-AuNPr-based fluorescence assay for highly sensitive and specific quantification of miRNAs in serum samples. Herein, we successfully synthesized and characterized fluorescently-labeled DNA-gold nanoparticle probes Nanoprobe via bioconjugation approach. In parallel, metaanalysis and qPCR analysis were performed on prostate tissues excised from PTEN PCa mouse model and on PCa patient serum to identified differential expression level of miRNAs. We also analyzed expression patterns of various miRNAs identified through meta-analysis of previously published reports in mouse PCa tissues and in PCa patient serum compare to their respective controls. We observed a significant overexpression of miR-21 and miR-375 in both case.

Subject Categories:

  • Medicine and Medical Research
  • Anatomy and Physiology
  • Biochemistry

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