Multifunctional PSCA antibody fragments for PET and optical prostate cancer imaging
Technical Report,30 Sep 2016,29 Sep 2017
University of California, Los Angeles Los Angeles United States
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The goals of this work are to develop engineered antibody fragments cys-diabodies and cys-minibodies for labeling with positron-emitting radionuclides and fluorescent dyes for multimodal imaging of prostate cancer. Previously, a novel cys-minibody recognizing PSCA prostate stem cell antigen was produced, conjugated, and radiolabeled with either I-124 or Zr-89. Targeting, imaging, and biodistribution studies in mouse models of prostate cancer confirmed excellent targeting and immunoPET imaging in PSCA-positive tumors in mice. This year, dual-labeling with radionuclide and fluorescent dye of the cys-minibody has been developed and evaluated in three different mouse models of prostate cancer. Furthermore, F-18 labeling has been established for a PSCA-specific cys-diabody and successfully imaged. A novel multifunctional linker has also been developed and dual F-18 PETNIR fluorescence imaging has been achieved.
- Medicine and Medical Research