Modeling Aggressive Medulloblastoma Using Human Induced Pluripotent Stem Cells
Technical Report,01 Jul 2014,30 Jun 2017
Hugo W. Moser Research Institute at Kennedy Krieger Inc. Baltimore United States
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In this project, we proposed to use neural cells derived from human induced pluripotent stem cells iPSCs to establish MYC-driven medulloblastoma MB model. This model will be used to identify the gene network regulated by MYC. We will also study the interaction between MYC and Atoh1, another essential transcription factor for MB development. Our results support that neural progenitors and neural stem cells induced from human induced pluripotent stem cells can be efficiently transformed by MYC oncogene to form aggressive brain tumors that recapitulate human group 3 MB. We further show that it is feasible to establish neurosphere cultures from these MB tumors to enrich brain tumor stem cells.
- Medicine and Medical Research