Testing of Ruggedized Antibodies within a Lateral Flow Immunoassay
Technical Report,01 Feb 2015,01 Sep 2015
Director, ECBC APG United States
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Antibody stability is a key quality parameter for a number of DoD customers who rely on effective and fielded hand-held immunoassays HHAs. This project leverages antibody ruggedization efforts at U.S. Army Edgewood Chemical BiologicalCenter ECBC to determine whether the ruggedization of detection antibodies translates into improved and more fieldable assays. Capitalizing on the work performed at ECBC in collaboration with the Defense Advanced Research Projects Agency,Antibody Technology Program and the Defense Threat Reduction Agency, Ruggedized Antibody Program, this effort was projected to develop a lateral flow immunoassay against the ricin toxin and increase thermostability that enlists the use of asmartCAR reader device, which is capable of communicating results with a smartphone. Incorporating previously ruggedized ricin antibodies into fieldable HHAs potentially makes these assays more amenable to forward-deployed hazard detection and eliminates or greatly reduces the current requirement for 4 C cold-chain logistics.
- Medicine and Medical Research
- Chemical, Biological and Radiological Warfare